Faouzi Laachari Lipase Activity

Faouzi Laachari Lipase Activity soby.in

Faouzi Laachari Lipase Activity. Isolation Purification And Characterization Of A Cold. Aspergillus nidulans WG312 strain secreted lipase activity when cultured in liquid media with olive oil as carbon source. Highest lipase productivity was found when the mycelium was grown at 30.

faouzi laachari lipase activity kirchenbezirk-nagold.de

Faouzi Laachari 1 Fatimazahra El Bergadi 12 Wifak Bahafid 12 Adel Sayari 3 Soumya Maximum lipase activity was found in the presence of Ca ions Olive oil and Tween 80 induced lipaseAs a leading global manufacturer of crushing equipment, milling equipment,dressing equipment,drying equipment and briquette equipment etc. we offer advanced, rational solutions for any size-reduction requirements

faouzi laachari lipase activity morleyiga.au

Lipase activity determination The lipase activity was measured titrimetrically at pH 8.2 and 45&# 76;C with a pH-stat under standard conditions using tributyrin (0.25 ml) in 30 ml of 2.5 mM Tris–HCl pH 8.2, 2 mM CaCl 2, 2 mM NaDC or olive oil emulsion ( 0 ml in Turk J Biochem 20 5; 40( ):8- 4 9 Laachari

Faouzi LAACHARI Faculté des Sciences et Techniques Fès

Faouzi Laachari, Soumya Elabed, Adel Sayari, Iraqui Mohammed, El Hassan Harchali, Saad Koraichi Ibnsouda. Biochemical characterization of a thermoactive and thermostable lipase from a newly olated

The Lipase from Staphylococcus aureus Simons 1996

Laachari Faouzi, El Bergad Fatimazahra, Sadiki Moulay, Sayari Adel, Bahafid Wifak, Elabed Soumya, Mohammed Iraqui, Koraichi Ibnsouda Saad, Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface, African Journal of Biochemistry Research, 10.5897/AJBR2014.0804, 9, 1, (9-17), (2015).

Biochemical characterization of a thermoactive and

Nonstop demand for greatly thermostable and thermoactive active lipase encourages the research for the new enzyme sources. In this study, a strain of Trichosporon coremiiforme was isolated from the traditional tannery in the city of Fez in Morocco, lipase production and their lipolytic activity was studied. Pure T. coremiiforme lipase (TCL) was obtained after ammonium sulfate fractionation, G

African Journal of Biochemistry Research higher

The main objective of this work was to identify novel lipases of industrial interest. In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC). Bile salts showed no inhibitory effect on the lipolytic activity, whereas

Faouzi LAACHARI Faculté des Sciences et Techniques Fès

Faouzi Laachari, Soumya Elabed, Adel Sayari, Iraqui Mohammed, El Hassan Harchali, Saad Koraichi Ibnsouda. Biochemical characterization of a thermoactive and thermostable lipase from a newly olated

The Lipase from Staphylococcus aureus Simons 1996

Laachari Faouzi, El Bergad Fatimazahra, Sadiki Moulay, Sayari Adel, Bahafid Wifak, Elabed Soumya, Mohammed Iraqui, Koraichi Ibnsouda Saad, Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface, African Journal of Biochemistry Research, 10.5897/AJBR2014.0804, 9, 1, (9-17), (2015).

Biochemical Characterization of a New Thermostable

The activity of lipase was examined within the pH range of 6.0-10.0 and the effect of pH on lipase stability was determined by All content in this area was uploaded by Faouzi Laachari on Mar

Biochemical characterization of a thermoactive and

Nonstop demand for greatly thermostable and thermoactive active lipase encourages the research for the new enzyme sources. In this study, a strain of Trichosporon coremiiforme was isolated from the traditional tannery in the city of Fez in Morocco, lipase production and their lipolytic activity was studied. Pure T. coremiiforme lipase (TCL) was obtained after ammonium sulfate fractionation, G

Lipases: Sources, Production, Purification, and

Laachari F, El Bergad F, Sadiki M, Higaki S, Kitagawa T, Kagoura M, et al. Correlation between Propionibacterium acnes biotypes, lipase activity and rash degree in acne patients. J Dermatol 2000; 27: 519-22. [121] Simons JWF, Adams H, Cox RC, et al.

African Journal of Biochemistry Research higher

The main objective of this work was to identify novel lipases of industrial interest. In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC). Bile salts showed no inhibitory effect on the lipolytic activity, whereas

Scilit Article Higher tolerance of a novel lipase from

The main objective of this work was to identify novel lipases of industrial interest. In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC). Bile salts showed no inhibitory effect on the lipolytic activity, whereas

Biochemical characterization of a new thermostable lipase

The activity of lipase was examined within the pH range of 6.0-10.0 and the effect of pH on lipase stability was determined by incubating the lipase fraction in various buffer solutions ranging from 3.0 to 10.0 for 24 h at room temperature. Results: The results showed that Bacillus pumilus is a strain that produce non-inducible lipase.

Isolement des microorganismes producteurs de lipases à

-Pr. Saad IBNSOUDA KORAICHI -Mr. Faouzi LAACHARI Soutenu le : 27 juin 2015 devant le jury composé de :-Pr. Saad IBNSOUDA Mes remerciements s’adressent également à Monsieur F.LAACHARI, Docteur pour l’aide, la compréhension, Mise en évidence de l’activité lipase-----19.

Lipase from Bacillus pumilus RK31: Production

A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange

The Lipase from Staphylococcus aureus Simons 1996

Laachari Faouzi, El Bergad Fatimazahra, Sadiki Moulay, Sayari Adel, Bahafid Wifak, Elabed Soumya, Mohammed Iraqui, Koraichi Ibnsouda Saad, Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface, African Journal of Biochemistry Research, 10.5897/AJBR2014.0804, 9, 1, (9-17), (2015).

Lipases: Sources, Production, Purification, and

Laachari F, El Bergad F, Sadiki M, Higaki S, Kitagawa T, Kagoura M, et al. Correlation between Propionibacterium acnes biotypes, lipase activity and rash degree in acne patients. J Dermatol 2000; 27: 519-22. [121] Simons JWF, Adams H, Cox RC, et al.

Medwin Publishers

Purification and Biochemical Characterization of a Lipase Produced by Aspergillus Niger: Interfacial Binding, Activity of Lipase at the Lipid-Water Interface Using Monomolecular Film Techniques. Faouzi Laachari* and Ibnsouda SK. PDF. Research Article.

Scilit Article Higher tolerance of a novel lipase from

The main objective of this work was to identify novel lipases of industrial interest. In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC). Bile salts showed no inhibitory effect on the lipolytic activity, whereas

Isolement des microorganismes producteurs de lipases à

-Pr. Saad IBNSOUDA KORAICHI -Mr. Faouzi LAACHARI Soutenu le : 27 juin 2015 devant le jury composé de :-Pr. Saad IBNSOUDA Mes remerciements s’adressent également à Monsieur F.LAACHARI, Docteur pour l’aide, la compréhension, Mise en évidence de l’activité lipase-----19.

Cellulolytic potential and filter paper activity of fungi

A total of 31 filamentous fungi were isolated from deteriorated paper of ancient books. Nine of these isolates were screened positive for the ability to degrade carboxymethylcellulose (CMC). The ability of the nine isolates to produce filter paper activity (FPUase) in liquid media as well as the effect of temperature and pH were also studied.

Realm of Thermoalkaline Lipases in Bioprocess Commodities

The TA lipase production by recombinant Pichia pastoris X33 harbouring lipase V3 gene can reach maximum lipase activity in fed-batch cultivation after 168 h induction with MeOH in STB [83, 91]. Meanwhile, TA lipase produced by T. harzianum IDM14D reached its maximum activity (0.24 U/mL) and biomass concentration (1.25 g/L) in SFC at 30°C for 7 d fermentation time [ 47 ].

Bacterial lipases: A review on purification and

01-01-2018· Lipase isolated from Bacillus sp. showed high activity towards oleic rich oils. Lipase immobilized on celite could retain 90% lipase activity after eight cycles. Trans-esterification of oil using the immobilized lipase obtained from Botryococcus sp. resulted in 80% yield of fatty acid methyl esters which had good properties for use as biodiesel ( Sivaramakrishnan and Incharoensakdi, 2016 ).

Cellulolytic potential and filter paper activity of fungi

The content of libraries in Fez is an important historical testimony and a treasure of knowledge of several civilizations. Unfortunately, this cultural heritage has suffered deterioration caused by many factors, including microbial deterioration specifically by cellulolytic fungi. The aim of our study was the identification and characterization of the microflora that damage historical

Lipase from Bacillus pumilus RK31: Production

A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange

Lipases: Sources, Production, Purification, and

Laachari F, El Bergad F, Sadiki M, Higaki S, Kitagawa T, Kagoura M, et al. Correlation between Propionibacterium acnes biotypes, lipase activity and rash degree in acne patients. J Dermatol 2000; 27: 519-22. [121] Simons JWF, Adams H, Cox RC, et al.

Bacterial lipases: A review on purification and

01-01-2018· Lipase isolated from Bacillus sp. showed high activity towards oleic rich oils. Lipase immobilized on celite could retain 90% lipase activity after eight cycles. Trans-esterification of oil using the immobilized lipase obtained from Botryococcus sp. resulted in 80% yield of fatty acid methyl esters which had good properties for use as biodiesel ( Sivaramakrishnan and Incharoensakdi, 2016 ).

Medwin Publishers

Purification and Biochemical Characterization of a Lipase Produced by Aspergillus Niger: Interfacial Binding, Activity of Lipase at the Lipid-Water Interface Using Monomolecular Film Techniques. Faouzi Laachari* and Ibnsouda SK. PDF. Research Article.

COMPARATIVE STUDIES ON THE KINETIC PROPERTIES OF

COMPARATIVE STUDIES ON THE KINETIC PROPERTIES OF LIPASE ISOLATED FROM Aspergillus nidulans AND Aspergillus niger. Home BIOCHEMISTRY PROJECT TOPICS AND MATERIALS COMPARATIVE STUDIES ON THE KINETIC PROPERTIES OF LIPASE ISOLATED FROM Aspergillus nidulans AND Aspergillus niger

Culture of Staphylococcus xylosus in fish processing by

The lipase activity was also measured during the growth time course. Experiments were conducted in duplicate. Lipase activity determination. The lipase activity was measured titrimetrically at pH 8·2 and 37°C with a pH‐stat under standard conditions using olive oil emulsion (10 ml of olive oil and 90 ml of Arabic gum 10%) as substrate.

Realm of Thermoalkaline Lipases in Bioprocess Commodities

The TA lipase production by recombinant Pichia pastoris X33 harbouring lipase V3 gene can reach maximum lipase activity in fed-batch cultivation after 168 h induction with MeOH in STB [83, 91]. Meanwhile, TA lipase produced by T. harzianum IDM14D reached its maximum activity (0.24 U/mL) and biomass concentration (1.25 g/L) in SFC at 30°C for 7 d fermentation time [ 47 ].

Isolation, Purification, and Characterization of a Cold

Aspergillus nidulans WG312 strain secreted lipase activity when cultured in liquid media with olive oil as carbon source. Highest lipase productivity was found when the mycelium was grown at 30 °C in a rich medium. The new enzyme was purified to homogeneity from the extracellular culture of A. nidulans by phenyl-Sepharose chromatography and affinity binding on linolenic acid−agarose.

Lipase from Bacillus pumilus RK31: Production

A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange

Cellulolytic potential and filter paper activity of fungi

The content of libraries in Fez is an important historical testimony and a treasure of knowledge of several civilizations. Unfortunately, this cultural heritage has suffered deterioration caused by many factors, including microbial deterioration specifically by cellulolytic fungi. The aim of our study was the identification and characterization of the microflora that damage historical

Ferritin regulates organismal energy balance and

Results. Under standard nutritional Fe supply, Fth deletion in adult Fth R26Δ/Δ mice led to a profound deregulation of organismal Fe metabolism, oxidative stress, inflammation, and multi-organ damage, culminating in death. Unexpectedly, Fth deletion was also associated with a profound atrophy of white and brown adipose tissue as well as with collapse of energy expenditure and thermogenesis.